YAF2 antibody

Catalog No. ABIN2461508

The Rabbit Polyclonal anti-YAF2 antibody is suitable to detect YAF2 in samples from Mouse. It has been validated for WB and ELISA.

Quick Overview for YAF2 antibody (ABIN2461508)

Target: YAF2 (YY1 Associated Factor 2 (YAF2))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This YAF2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-YAF2 Antibody

Purification: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of mouse YAF2.

Application Details

Application Notes: YAF2 antibody can be used for detection of YAF2 by ELISA at 1:312500. YAF2 antibody can be used for detection of YAF2 by western blot at 0.2 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store YAF2 antibody at -20 °C.

Target Details for YAF2

Target: YAF2 (YY1 Associated Factor 2 (YAF2))

Alternative Name: YAF2

Background: Yaf2 binds to MYC and inhibits MYC-mediated transactivation. Yaf2 also binds to MYCN and enhances MYCN-dependent transcriptional activation. Yaf2 increases calpain 2-mediated proteolysis of YY1 in vitro. Yaf2 is a component of the E2F6.com-1 complex, a repressive complex that methylates Lys-9 of histone H3, suggesting that it is involved in chromatin-remodeling.

Molecular Weight: 20 kDa

Gene ID: 67057

NCBI Accession: NP_077151

UniProt: Q99LW6