Actin alpha 2 antibody
The Rabbit Polyclonal anti-Actin alpha 2 antibody (ABIN2461527) specifically detects Actin alpha 2 in ELISA and WB.
The antibody is reactive with Mouse and Rat samples.
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Catalog No. ABIN2461527
$650.38
Plus shipping costs $50.00
100 μL
Shipping to:
United States
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Quick Overview for Actin alpha 2 antibody (ABIN2461527)
Target
Actin alpha 2
Reactivity
Mouse, Rat
Host
Rabbit
Clonality
Polyclonal
Conjugate
Un-conjugated
Application
ELISA, Western Blotting (WB)
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Purification
- Antibody is purified by protein A chromatography method.
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Immunogen
- Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of mouse Actin alpha 2.
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anti-Actin alpha 2 antibody
Reactivity: Mouse, Rat, Dog, Human ELISA, WB, IHC Host: Rabbit Polyclonal unconjugated
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Application Notes
- Actin alpha 2 antibody can be used for detection of Actin alpha 2 by ELISA at 1:312500. Actin alpha 2 antibody can be used for detection of Actin alpha 2 by western blot at 0.625 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.
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Concentration
- 1 mg/mL
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Buffer
- Antibody is lyophilized in PBS buffer with 2 % sucrose.
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Handling Advice
- As with any antibody avoid repeat freeze-thaw cycles.
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Storage
- 4 °C/-20 °C
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Storage Comment
- For short periods of storage (days) store at 4 °C. For longer periods of storage, store Actin alpha 2 antibody at -20 °C.
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- Actin alpha 2
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Background
- The alpha-actinins are a multigene family of four actin-binding proteins related to dystrophin. The two skeletal muscle isoforms of alpha-actinin (ACTN2 and ACTN3) are major structural components of the Z-line involved in anchoring the actin-containing thin filaments. In humans, ACTN2 is expressed in all muscle fibres, while ACTN3 expression is restricted to a subset of type 2 fibres. Murine Actn2 and Actn3 are differentially expressed, spatially and temporally, during embryonic development and, in contrast to humans, alpha-actinin-2 expression does not completely overlap alpha-actinin-3 in postnatal skeletal muscle, suggesting independent function.
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Molecular Weight
- 98 kDa
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Gene ID
- 11472
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NCBI Accession
- NP_150371
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UniProt
- Q9JI91
Target
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