ZNF265 antibody

Catalog No. ABIN2461594

This Rabbit Polyclonal antibody specifically detects ZNF265 in WB and ELISA. It exhibits reactivity toward Human, Mouse, Rat, Dog and Zebrafish (Danio rerio).

Quick Overview for ZNF265 antibody (ABIN2461594)

Target: ZNF265 (Zranb2) (Zinc Finger Protein 265 (Zranb2))

Reactivity: Human, Mouse, Rat, Dog, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ZNF265 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-ZNF265 Antibody

Purification: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ZNF265.

Application Details

Application Notes: ZNF265 antibody can be used for detection of ZNF265 by ELISA at 1:312500. ZNF265 antibody can be used for detection of ZNF265 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store ZNF265 antibody at -20 °C.

Target Details for ZNF265

Target: ZNF265 (Zranb2) (Zinc Finger Protein 265 (Zranb2))

Alternative Name: ZNF265

Background: ZNF265 is a protein that has been shown to bind to the spliceosomal components U1-70K and U2AF35 and to direct alternative splicing. Analysis of the structure reveals substantial similarity to known RNA-binding motifs in terms of the distribution of key surface residues responsible for making RNA contacts, despite a complete lack of structural homology. An RNA gel shift assay was used to demonstrate that a single crossed finger domain from ZNF265 is capable of binding to an RNA message. Taken together, these results define a new RNA-binding motif and should provide insight into the functions of the >100 uncharacterized proteins in the sequence data bases that contain this domain.

Molecular Weight: 37 kDa, 36 kDa

Gene ID: 9406

NCBI Accession: NP_976225

UniProt: Q5VV34