TIMELESS antibody

Catalog No. ABIN2461712

The Rabbit Polyclonal anti-TIMELESS antibody has been validated for WB and ELISA. It is suitable to detect TIMELESS in samples from Human and Dog.

Quick Overview for TIMELESS antibody (ABIN2461712)

Target: TIMELESS (Timeless Homolog (TIMELESS))

Reactivity: Human, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TIMELESS antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-TIMELESS Antibody

Purification: Antibody is purified by protein A chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human TIMELESS.

Application Details

Application Notes: TIMELESS antibody can be used for detection of TIMELESS by ELISA at 1:312500. TIMELESS antibody can be used for detection of TIMELESS by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store TIMELESS antibody at -20 °C.

Target Details for TIMELESS

Target: TIMELESS (Timeless Homolog (TIMELESS))

Alternative Name: TIMELESS

Background: The human Timeless protein interacts with both the circadian clock protein cryptochrome 2 and with the cell cycle checkpoint proteins Chk1 and the ATR-ATRIP complex and plays an important role in the DNA damage checkpoint response. Down-regulation of Timeless in human cells seriously compromises replication and intra-S checkpoints, indicating an intimate connection between the circadian cycle and the DNA damage checkpoints that is in part mediated by the Timeless protein.

Molecular Weight: 139 kDa

Gene ID: 8914

NCBI Accession: NP_003911

UniProt: Q9UNS1

Pathways: Protein targeting to Nucleus, Photoperiodism