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MED27 antibody

This Rabbit Polyclonal antibody specifically detects MED27 in ELISA and WB. It exhibits reactivity toward Human, Mouse, Dog and Rat.
Catalog No. ABIN2461720

Quick Overview for MED27 antibody (ABIN2461720)

Target

See all MED27 Antibodies
MED27 (Mediator Complex Subunit 27 (MED27))

Reactivity

  • 33
  • 15
  • 5
  • 4
  • 4
  • 4
  • 4
  • 3
  • 3
  • 2
  • 2
  • 2
  • 1
  • 1
Human, Mouse, Dog, Rat

Host

  • 30
  • 4
Rabbit

Clonality

  • 32
  • 2
Polyclonal

Conjugate

  • 20
  • 3
  • 3
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This MED27 antibody is un-conjugated

Application

  • 18
  • 14
  • 3
  • 2
  • 1
  • 1
ELISA, Western Blotting (WB)
  • Purification

    Antibody is purified by protein A chromatography method.

    Immunogen

    Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CRSP8.
  • Application Notes

    CRSP8 antibody can be used for detection of CRSP8 by ELISA at 1:62500. CRSP8 antibody can be used for detection of CRSP8 by western blot at 5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.

    Concentration

    1 mg/mL

    Buffer

    Antibody is lyophilized in PBS buffer with 2 % sucrose.

    Handling Advice

    As with any antibody avoid repeat freeze-thaw cycles.

    Storage

    4 °C/-20 °C

    Storage Comment

    For short periods of storage (days) store at 4 °C. For longer periods of storage, store CRSP8 antibody at -20 °C.
  • Target

    MED27 (Mediator Complex Subunit 27 (MED27))

    Alternative Name

    CRSP8

    Background

    He activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. CRSP8 is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. CRSP8 is also a component of other multisubunit complexes e.g. thyroid hormone receptor- (TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors.The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor- (TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors.

    Molecular Weight

    35 kDa

    Gene ID

    9442

    NCBI Accession

    NP_004260

    UniProt

    Q6P2C8

    Pathways

    Stem Cell Maintenance, Regulation of Lipid Metabolism by PPARalpha
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