MED27 antibody

Catalog No. ABIN2461720

This Rabbit Polyclonal antibody specifically detects MED27 in WB and ELISA. It exhibits reactivity toward Human, Mouse, Dog and Rat.

Quick Overview for MED27 antibody (ABIN2461720)

Target: MED27 (Mediator Complex Subunit 27 (MED27))

Reactivity: Human, Mouse, Dog, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MED27 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-MED27 Antibody

Purification: Antibody is purified by protein A chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CRSP8.

Application Details

Application Notes: CRSP8 antibody can be used for detection of CRSP8 by ELISA at 1:62500. CRSP8 antibody can be used for detection of CRSP8 by western blot at 5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store CRSP8 antibody at -20 °C.

Target Details for MED27

Target: MED27 (Mediator Complex Subunit 27 (MED27))

Alternative Name: CRSP8

Background: He activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. CRSP8 is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. CRSP8 is also a component of other multisubunit complexes e.g. thyroid hormone receptor- (TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors.The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor- (TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors.

Molecular Weight: 35 kDa

Gene ID: 9442

NCBI Accession: NP_004260

UniProt: Q6P2C8

Pathways: Stem Cell Maintenance, Regulation of Lipid Metabolism by PPARalpha