MED27 antibody
Quick Overview for MED27 antibody (ABIN2461720)
Target
See all MED27 AntibodiesReactivity
Host
Clonality
Conjugate
Application
-
-
Purification
- Antibody is purified by protein A chromatography method.
-
Immunogen
- Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CRSP8.
-
-
-
-
Application Notes
- CRSP8 antibody can be used for detection of CRSP8 by ELISA at 1:62500. CRSP8 antibody can be used for detection of CRSP8 by western blot at 5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
-
Restrictions
- For Research Use only
-
-
-
Format
- Lyophilized
-
Reconstitution
- Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.
-
Concentration
- 1 mg/mL
-
Buffer
- Antibody is lyophilized in PBS buffer with 2 % sucrose.
-
Handling Advice
- As with any antibody avoid repeat freeze-thaw cycles.
-
Storage
- 4 °C/-20 °C
-
Storage Comment
- For short periods of storage (days) store at 4 °C. For longer periods of storage, store CRSP8 antibody at -20 °C.
-
-
- MED27 (Mediator Complex Subunit 27 (MED27))
-
Alternative Name
- CRSP8
-
Background
- He activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. CRSP8 is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. CRSP8 is also a component of other multisubunit complexes e.g. thyroid hormone receptor- (TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors.The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor- (TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors.
-
Molecular Weight
- 35 kDa
-
Gene ID
- 9442
-
NCBI Accession
- NP_004260
-
UniProt
- Q6P2C8
-
Pathways
- Stem Cell Maintenance, Regulation of Lipid Metabolism by PPARalpha
Target
-