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MED27 antibody (Mediator Complex Subunit 27) (Middle Region) Primary Antibody

MED27 Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit WB Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN2778099
$389.00
Plus shipping costs $45.00
100 μL ABIN2778099
100 μL ABIN2778099
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  • Target
    MED27
    Binding Specificity
    • 4
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Middle Region
    Reactivity
    • 27
    • 16
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit
    Host
    • 23
    • 4
    Rabbit
    Clonality
    • 25
    • 2
    Polyclonal
    Conjugate
    • 17
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This MED27 antibody is un-conjugated
    Application
    • 19
    • 11
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB)
    Sequence
    LSRPNGTSAM LLVTLGKVLK VIVVMRSLFI DRTIVKGYNE NVYTEDGKLD
    Predicted Reactivity
    Cow: 100%, Dog: 85%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 92%, Rabbit: 85%, Rat: 100%
    Characteristics
    This is a rabbit polyclonal antibody against MED27. It was validated on Western Blot using a cell lysate as a positive control.
    Purification
    Affinity Purified
    Immunogen
    The immunogen is a synthetic peptide directed towards the middle region of human MED27
  • Application Notes
    Optimal working dilutions should be determined experimentally by the investigator.
    Comment

    Antigen size: 311 AA

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -20 °C
    Storage Comment
    For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
  • Target
    MED27
    Alternative Name
    MED27 (MED27 Antibody Abstract)
    Synonyms
    CRAP34, CRSP34, CRSP8, TRAP37, CG1245, Dmel\\CG1245, Med27, Trap37, dCRSP34, dCrsp34, dTRAP37, med27, crsp8, zgc:66302, MED27, DDBDRAFT_0216764, DDBDRAFT_0266847, DDB_0216764, DDB_0266847, 1500015J03Rik, 2310042P07Rik, AA682045, Crsp8, D2Ertd434e, RGD1564993, DKFZp459I1912, CRSP complex subunit 8-B, Xcrsp34, crap34, crsp34, crsp8-B, med27-a, med27-b, trap37, mediator complex subunit 27, Mediator complex subunit 27, cofactor required for Sp1 transcriptional activation, subunit 8, 34kDa, hypothetical protein, mediator complex subunit 27 S homeolog, MED27, med27, crsp8, Med27, med27.S
    Background
    The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. MED27 is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors.The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors.
    Alias Symbols: CRAP34, CRSP34, CRSP8, MGC11274, TRAP37
    Protein Interaction Partner: UBC, CDK19, CDK8, MED19, ARFGAP1, MED26, SOX5, POLR2C, RBPJ, AFP, FBXW7, EPAS1, MED4, MED12, MED14, MED1, MED28, OBFC1, MED18, ZC3H13, QKI, TRIP4, TADA2A, MED9, MED30, MED8, MED22, MED10, MED29,
    Protein Size: 311
    Molecular Weight
    35 kDa
    Gene ID
    9442
    NCBI Accession
    NM_004269, NP_004260
    UniProt
    Q6P2C8
    Pathways
    Stem Cell Maintenance, Regulation of Lipid Metabolism by PPARalpha
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