CTCF antibody

Catalog No. ABIN2461796

The Rabbit Polyclonal anti-CTCF antibody has been validated for WB and ELISA. It is suitable to detect CTCF in samples from Human, Mouse, Rat and Dog.

Quick Overview for CTCF antibody (ABIN2461796)

Target: CTCF (CCCTC-Binding Factor (Zinc Finger Protein) (CTCF))

Reactivity: Human, Mouse, Rat, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CTCF antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-CTCF Antibody

Purification: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CTCF.

Application Details

Application Notes: CTCF antibody can be used for detection of CTCF by ELISA at 1:312500. CTCF antibody can be used for detection of CTCF by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store CTCF antibody at -20 °C.

Target Details for CTCF

Target: CTCF (CCCTC-Binding Factor (Zinc Finger Protein) (CTCF))

Alternative Name: CTCF

Background: CTCF is a transcriptional regulator protein with 11 highly conserved zinc finger (ZF) domains. This nuclear protein is able to use different combinations of the ZF domains to bind different DNA target sequences and proteins. Depending upon the context of the site, the protein can bind a histone acetyltransferase (HAT)-containing complex and function as a transcriptional activator or bind a histone deacetylase (HDAC)-containing complex and function as a transcriptional repressor. If the protein is bound to a transcriptional insulator element, it can block communication between enhancers and upstream promoters, thereby regulating imprinted expression. Mutations in CTCF have been associated with invasive breast cancers, prostate cancers, and Wilms' tumors.This gene is a member of the BORIS + CTCF gene family and encodes a transcriptional regulator protein with 11 highly conserved zinc finger (ZF) domains. This nuclear protein is able to use different combinations of the ZF domains to bind different DNA target sequences and proteins. Depending upon the context of the site, the protein can bind a histone acetyltransferase (HAT)-containing complex and function as a transcriptional activator or bind a histone deacetylase (HDAC)-containing complex and function as a transcriptional repressor. If the protein is bound to a transcriptional insulator element, it can block communication between enhancers and upstream promoters, thereby regulating imprinted expression. Mutations in this gene have been associated with invasive breast cancers, prostate cancers, and Wilms' tumors.

Molecular Weight: 83 kDa

Gene ID: 10664

NCBI Accession: NP_006556

UniProt: P49711