RAE1 antibody

Catalog No. ABIN2462089

This Rabbit Polyclonal antibody specifically detects RAE1 in WB, IHC and ELISA. It exhibits reactivity toward Human, Mouse, Rat and Dog.

Quick Overview for RAE1 antibody (ABIN2462089)

Target: RAE1 (RAE1 RNA Export 1 Homolog (S. Pombe) (RAE1))

Reactivity: Human, Mouse, Rat, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAE1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-RAE1 Antibody

Purification: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human RAE1.

Application Details

Application Notes: RAE1 antibody can be used for detection of RAE1 by ELISA at 1:312500. RAE1 antibody can be used for detection of RAE1 by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store RAE1 antibody at -20 °C.

Target Details for RAE1

Target: RAE1 (RAE1 RNA Export 1 Homolog (S. Pombe) (RAE1))

Alternative Name: RAE1

Background: RAE1 is a homolog of yeast Rae1. It contains four WD40 motifs, and has been shown to localize to distinct foci in the nucleoplasm, to the nuclear rim, and to meshwork-like structures throughout the cytoplasm. This gene is thought to be involved in nucleocytoplasmic transport, and in directly or indirectly attaching cytoplasmic mRNPs to the cytoskeleton.Mutations in the Schizosaccharomyces pombe Rae1 and Saccharomyces cerevisiae Gle2 genes have been shown to result in accumulation of poly (A)-containing mRNA in the nucleus, suggesting that the encoded proteins are involved in RNA export. The protein encoded by this gene is a homolog of yeast Rae1. It contains four WD40 motifs, and has been shown to localize to distinct foci in the nucleoplasm, to the nuclear rim, and to meshwork-like structures throughout the cytoplasm. This gene is thought to be involved in nucleocytoplasmic transport, and in directly or indirectly attaching cytoplasmic mRNPs to the cytoskeleton. Alternatively spliced transcript variants encoding the same protein have been found for this gene.

Molecular Weight: 40 kDa, 41 kDa

Gene ID: 8480

NCBI Accession: NP_001015885

UniProt: P78406

Pathways: SARS-CoV-2 Protein Interactome