Nucleolin antibody

Catalog No. ABIN2462187

This anti-Nucleolin antibody is a Rabbit Polyclonal antibody detecting Nucleolin in WB, IHC and ELISA. Suitable for Human, Rat and Dog.

Quick Overview for Nucleolin antibody (ABIN2462187)

Target: Nucleolin (NCL)

Reactivity: Human, Rat, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nucleolin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-Nucleolin Antibody

Purification: Antibody is purified by protein A chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NCL.

Application Details

Application Notes: NCL antibody can be used for detection of NCL by ELISA at 1:312500. NCL antibody can be used for detection of NCL by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store NCL antibody at -20 °C.

Target Details for Nucleolin

Target: Nucleolin (NCL)

Alternative Name: NCL

Background: Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus.Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20.

Molecular Weight: 78 kDa

Gene ID: 4691

NCBI Accession: NP_005372

UniProt: P19338

Pathways: Ribonucleoprotein Complex Subunit Organization