This Rabbit Polyclonal antibody specifically detects CPSF3 in WB and ELISA. It exhibits reactivity toward Human, Mouse, Rat, Dog and Zebrafish (Danio rerio).
CPSF3 antibody can be used for detection of CPSF3 by ELISA at 1:312500. CPSF3 antibody can be used for detection of CPSF3 by western blot at 5.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.
Concentration
1 mg/mL
Buffer
Antibody is lyophilized in PBS buffer with 2 % sucrose.
Handling Advice
As with any antibody avoid repeat freeze-thaw cycles.
Storage
4 °C/-20 °C
Storage Comment
For short periods of storage (days) store at 4 °C. For longer periods of storage, store CPSF3 antibody at -20 °C.
Target
CPSF3
(Cleavage and Polyadenylation Specific Factor 3, 73kDa (CPSF3))
Alternative Name
CPSF3
Background
CPSF3 belongs to the RNA-metabolizing metallo-beta-lactamase-like family, CPSF3 subfamily. It is component of the cleavage and polyadenylation specificity factor (CPSF) complex that play a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly (A) polymerase and other factors to bring about cleavage and poly (A) addition.