TNNI1 antibody

Catalog No. ABIN2462531

The Rabbit Polyclonal anti-TNNI1 antibody has been validated for WB and ELISA. It is suitable to detect TNNI1 in samples from Human, Rat, Mouse and Zebrafish (Danio rerio).

Quick Overview for TNNI1 antibody (ABIN2462531)

Target: TNNI1 (Slow Skeletal Troponin I (TNNI1))

Reactivity: Human, Rat, Mouse, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TNNI1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-TNNI1 Antibody

Purification: Antibody is purified by protein A chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human TNNI1.

Application Details

Application Notes: TNNI1 antibody can be used for detection of TNNI1 by ELISA at 1:62500. TNNI1 antibody can be used for detection of TNNI1 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store TNNI1 antibody at -20 °C.

Target Details for TNNI1

Target: TNNI1 (Slow Skeletal Troponin I (TNNI1))

Alternative Name: TNNI1

Background: Troponin proteins associate with tropomyosin and regulate the calcium sensitivity of the myofibril contractile apparatus of striated muscles. Each troponin-tropomyosin complex contains 1 Molecule from each of the 3 troponins: T, C, and I. The troponin I (TNI) subfamily contains three genes: TNI-skeletal-fast-twitch, TNI-skeletal-slow-twitch, and TNI-cardiac. TNNI1 prevents muscle contraction by inhibiting calcium-mediated conformational changes in actin-myosin complexes.

Molecular Weight: 21 kDa

Gene ID: 7135

NCBI Accession: NP_003272

UniProt: P19237