PPAP2A antibody

Catalog No. ABIN2462534

The Rabbit Polyclonal anti-PPAP2A antibody has been validated for WB and ELISA. It is suitable to detect PPAP2A in samples from Human, Mouse and Zebrafish (Danio rerio).

Quick Overview for PPAP2A antibody (ABIN2462534)

Target: PPAP2A (Phosphatidic Acid Phosphatase Type 2A (PPAP2A))

Reactivity: Human, Mouse, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PPAP2A antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-PPAP2A Antibody

Purification: Antibody is purified by protein A chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PPAP2A.

Application Details

Application Notes: PPAP2A antibody can be used for detection of PPAP2A by ELISA at 1:62500. PPAP2A antibody can be used for detection of PPAP2A by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store PPAP2A antibody at -20 °C.

Target Details for PPAP2A

Target: PPAP2A (Phosphatidic Acid Phosphatase Type 2A (PPAP2A))

Alternative Name: PPAP2A

Background: PPAP2A is a member of the phosphatidic acid phosphatase (PAP) family. PAPs convert phosphatidic acid to diacylglycerol, and function in de novo synthesis of glycerolipids as well as in receptor-activated signal transduction mediated by phospholipase D. This protein is an integral membrane glycoprotein, and has been shown to be a surface enzyme that plays an active role in the hydrolysis and uptake of lipids from extracellular space. The expression of PPAP2A is found to be regulated by androgen in a prostatic adenocarcinoma cell line.The protein encoded by this gene is a member of the phosphatidic acid phosphatase (PAP) family. PAPs convert phosphatidic acid to diacylglycerol, and function in de novo synthesis of glycerolipids as well as in receptor-activated signal transduction mediated by phospholipase D. This protein is an integral membrane glycoprotein, and has been shown to be a surface enzyme that plays an active role in the hydrolysis and uptake of lipids from extracellular space. The expression of this gene is found to be regulated by androgen in a prostatic adenocarcinoma cell line. At least two alternatively spliced transcript variants encoding distinct isoforms have been described.

Molecular Weight: 31 kDa, 32 kDa

Gene ID: 8611

NCBI Accession: NP_795714

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway