PPAP2A antibody (Middle Region)

Catalog No. ABIN2777163

The Rabbit Polyclonal anti-PPAP2A antibody (ABIN2777163) specifically detects PPAP2A in WB and IHC. The antibody is reactive with Human, Rat, Mouse, Guinea Pig, Dog, Horse, Zebrafish (Danio rerio), Cow and Rabbit samples.

Quick Overview for PPAP2A antibody (Middle Region) (ABIN2777163)

Target: PPAP2A (Phosphatidic Acid Phosphatase Type 2A (PPAP2A))

Reactivity: Human, Rat, Mouse, Guinea Pig, Dog, Horse, Zebrafish (Danio rerio), Cow, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PPAP2A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-PPAP2A Antibody

Binding Specificity: Middle Region

Sequence: DPDWSKINCS DGYIEYYICR GNAERVKEGR LSFYSGHSSF SMYCMLFVAL

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against PPAP2A. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human PPAP2A

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 285 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for PPAP2A

Target: PPAP2A (Phosphatidic Acid Phosphatase Type 2A (PPAP2A))

Alternative Name: PPAP2A

Background: PPAP2A is a member of the phosphatidic acid phosphatase (PAP) family. PAPs convert phosphatidic acid to diacylglycerol, and function in de novo synthesis of glycerolipids as well as in receptor-activated signal transduction mediated by phospholipase D. This protein is an integral membrane glycoprotein, and has been shown to be a surface enzyme that plays an active role in the hydrolysis and uptake of lipids from extracellular space. The expression of PPAP2A is found to be regulated by androgen in a prostatic adenocarcinoma cell line.The protein encoded by this gene is a member of the phosphatidic acid phosphatase (PAP) family. PAPs convert phosphatidic acid to diacylglycerol, and function in de novo synthesis of glycerolipids as well as in receptor-activated signal transduction mediated by phospholipase D. This protein is an integral membrane glycoprotein, and has been shown to be a surface enzyme that plays an active role in the hydrolysis and uptake of lipids from extracellular space. The expression of this gene is found to be regulated by androgen in a prostatic adenocarcinoma cell line. At least two alternatively spliced transcript variants encoding distinct isoforms have been described.
Alias Symbols: LLP1a, LPP1, PAP-2a, PAP2, PAP2a2, PAP2alpha2, PAPalpha1
Protein Interaction Partner: FAHD1, IGF2BP2, ILF3, UBC, FXYD1,
Protein Size: 285

Molecular Weight: 31 kDa

Gene ID: 8611

NCBI Accession: NM_176895, NP_795714

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway