RGS18 antibody

Catalog No. ABIN2462635

This Rabbit Polyclonal antibody specifically detects RGS18 in ELISA and WB. It exhibits reactivity toward Human and Dog.

Quick Overview for RGS18 antibody (ABIN2462635)

Target: RGS18 (Regulator of G-Protein Signalling 18 (RGS18))

Reactivity: Human, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RGS18 antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Product Details anti-RGS18 Antibody

Purification: Antibody is purified by protein A chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human RGS18.

Application Details

Application Notes: RGS18 antibody can be used for detection of RGS18 by ELISA at 1:62500. RGS18 antibody can be used for detection of RGS18 by western blot at 5.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store RGS18 antibody at -20 °C.

Target Details for RGS18

Target: RGS18 (Regulator of G-Protein Signalling 18 (RGS18))

Alternative Name: RGS18

Background: RGS18 is a member of the regulator of G-protein signaling family. This protein is contains a conserved, 120 amino acid motif called the RGS domain. The protein attenuates the signaling activity of G-proteins by binding to activated, GTP-bound G alpha subunits and acting as a GTPase activating protein (GAP), increasing the rate of conversion of the GTP to GDP. This hydrolysis allows the G alpha subunits to bind G beta/gamma subunit heterodimers, forming inactive G-protein heterotrimers, thereby terminating the signal.This gene encodes a member of the regulator of G-protein signaling family. This protein is contains a conserved, 120 amino acid motif called the RGS domain. The protein attenuates the signaling activity of G-proteins by binding to activated, GTP-bound G alpha subunits and acting as a GTPase activating protein (GAP), increasing the rate of conversion of the GTP to GDP. This hydrolysis allows the G alpha subunits to bind G beta/gamma subunit heterodimers, forming inactive G-protein heterotrimers, thereby terminating the signal. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized.

Molecular Weight: 26 kDa

Gene ID: 64407

NCBI Accession: NP_570138

UniProt: Q9NS28

Pathways: Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling