RNF40 antibody

Catalog No. ABIN2462672

The Rabbit Polyclonal anti-RNF40 antibody has been validated for WB, ELISA and IHC. It is suitable to detect RNF40 in samples from Human, Mouse, Rat and Dog.

Quick Overview for RNF40 antibody (ABIN2462672)

Target: RNF40 (Ring Finger Protein 40, E3 Ubiquitin Protein Ligase (RNF40))

Reactivity: Human, Mouse, Rat, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RNF40 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-RNF40 Antibody

Purification: Antibody is purified by protein A chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human RNF40.

Application Details

Application Notes: RNF40 antibody can be used for detection of RNF40 by ELISA at 1:312500. RNF40 antibody can be used for detection of RNF40 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store RNF40 antibody at -20 °C.

Target Details for RNF40

Target: RNF40 (Ring Finger Protein 40, E3 Ubiquitin Protein Ligase (RNF40))

Alternative Name: RNF40

Background: RNF40 contains a RING finger, a motif known to be involved in protein-protein and protein-DNA interactions. This protein was reported to interact with the tumor suppressor protein RB1. Studies of the rat counterpart suggested that this protein may function as an E3 ubiquitin-protein ligase, and facilitate the ubiquitination and degradation of syntaxin 1, which is an essential component of the neurotransmitter release machinery.The protein encoded by this gene contains a RING finger, a motif known to be involved in protein-protein and protein-DNA interactions. This protein was reported to interact with the tumor suppressor protein RB1. Studies of the rat counterpart suggested that this protein may function as an E3 ubiquitin-protein ligase, and facilitate the ubiquitination and degradation of syntaxin 1, which is an essential component of the neurotransmitter release machinery.

Molecular Weight: 114 kDa

Gene ID: 9810

NCBI Accession: NP_055586

UniProt: O75150