ALT antibody

Catalog No. ABIN2462887

This Rabbit Polyclonal antibody specifically detects ALT in WB, IHC and ELISA. It exhibits reactivity toward Human, Rat, Mouse and Dogand has been mentioned in 1 publication.

Quick Overview for ALT antibody (ABIN2462887)

Target: ALT (Alanine Aminotransferase (ALT))

Reactivity: Human, Rat, Mouse, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ALT antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-ALT Antibody

Purification: Antibody is purified by protein A chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human GPT.

Application Details

Application Notes: GPT antibody can be used for detection of GPT by ELISA at 1:312500. GPT antibody can be used for detection of GPT by western blot at 5.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store GPT antibody at -20 °C.

References for anti-ALT antibody (ABIN2462887)

Mousa, Soliman, Alhumaydhi, Almatroudi, Al Rugaie, Allemailem, Alrumaihi, Khan, Rezk, Aljasir, Alwashmi, Aba Alkhayl, Albutti, Seleem: "Garlic Extract Alleviates Trastuzumab-Induced Hepatotoxicity in Rats Through Its Antioxidant, Anti-Inflammatory, and Antihyperlipidemic Effects." in: Journal of inflammation research, Vol. 14, pp. 6305-6316, (2021) (PubMed).

Target Details for ALT

Target: ALT (Alanine Aminotransferase (ALT))

Alternative Name: GPT

Background: GPT participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles.

Molecular Weight: 55 kDa

Gene ID: 2875

NCBI Accession: NP_005300

UniProt: P24298