CAD antibody

Catalog No. ABIN2463103

This Rabbit Polyclonal antibody specifically detects CAD in WB and ELISA. It exhibits reactivity toward Drosophila melanogaster.

Quick Overview for CAD antibody (ABIN2463103)

Target: CAD (Carbamoyl-Phosphate Synthetase 2, Aspartate Transcarbamylase, and Dihydroorotase (CAD))

Reactivity: Drosophila melanogaster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CAD antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-CAD Antibody

Purification: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of Drosophila CAD.

Application Details

Application Notes: CAD antibody can be used for detection of CAD by ELISA at 1:12500. CAD antibody can be used for detection of CAD by western blot at 1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store CAD antibody at -20 °C.

Target Details for CAD

Target: CAD (Carbamoyl-Phosphate Synthetase 2, Aspartate Transcarbamylase, and Dihydroorotase (CAD))

Alternative Name: CAD

Background: Cad regulates embryonic abdominal segment formation by zygotically activating expression of knirps (kni) and giant (gt). It plays a role in the establishment of the hindgut and in the invagination of the hindgut primordium during gastrulation. These effects on the gut are achieved by acting combinatorially at the posterior of the embryo to activate transcription of different targets, including folded gastrulation (fog), fork head (fkh) and wingless (wg).

Molecular Weight: 46 kDa

Gene ID: 35341

NCBI Accession: NP_599128

UniProt: P09085

Pathways: Production of Molecular Mediator of Immune Response, Ribonucleoside Biosynthetic Process