14-3-3 zeta antibody

Catalog No. ABIN2463127

This Rabbit Polyclonal antibody specifically detects 14-3-3 zeta in WB and ELISA. It exhibits reactivity toward Human, Mouse, Rat and Dog.

Quick Overview for 14-3-3 zeta antibody (ABIN2463127)

Target: 14-3-3 zeta (YWHAZ)

Reactivity: Human, Mouse, Rat, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This 14-3-3 zeta antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-14-3-3 zeta Antibody

Purification: Antibody is purified by protein A chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human YWHAZ.

Application Details

Application Notes: YWHAZ antibody can be used for detection of YWHAZ by ELISA at 1:62500. YWHAZ antibody can be used for detection of YWHAZ by western blot at 5.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store YWHAZ antibody at -20 °C.

Target Details for 14-3-3 zeta

Target: 14-3-3 zeta (YWHAZ)

Alternative Name: YWHAZ

Background: YWHAZ belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99 % identical to the mouse, rat and sheep orthologs. The protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity.This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99 % identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Two transcript variants differing in the 5' UTR, but encoding the same protein, have been identified for this gene.

Molecular Weight: 28 kDa, 28 kDa

Gene ID: 7534

NCBI Accession: NP_003397

UniProt: P63104

Pathways: Apoptosis, Hormone Transport, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Synaptic Membrane, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location