Histone H3.3 antibody (N-Term)

Catalog No. ABIN2463650

The Rabbit Polyclonal anti-Histone H3.3 antibody is suitable to detect Histone H3.3 in samples from Human. It has been validated for WB and ELISA.

Quick Overview for Histone H3.3 antibody (N-Term) (ABIN2463650)

Target: Histone H3.3 (H3F3A) (H3 Histone, Family 3A (H3F3A))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone H3.3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-Histone H3.3 Antibody

Binding Specificity: N-Term

Purification: Antibody is purified by Protein A/G affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide directed towards the N terminal of human HIST3H3

Application Details

Application Notes: H3F3A antibody can be used for detection of H3F3A by ELISA at >1:3000. H3F3A antibody can be used for detection of H3F3A by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store H3F3A antibody at -20 °C.

Target Details for Histone H3.3

Target: Histone H3.3 (H3F3A) (H3 Histone, Family 3A (H3F3A))

Alternative Name: H3F3A

Background: Histone H3, along with histone H4, plays a central role in nucleosome formation.

Molecular Weight: 15 kDa, 15 kDa, 16 kDa, 15 kDa, 23 kDa

Gene ID: 8290

NCBI Accession: NP_002098

UniProt: P06351