14-3-3 theta antibody

Catalog No. ABIN2463667

This Rabbit Polyclonal antibody specifically detects 14-3-3 theta in WB, ELISA and IHC. It exhibits reactivity toward Human, Mouse, Rat and Dog.

Quick Overview for 14-3-3 theta antibody (ABIN2463667)

Target: 14-3-3 theta (YWHAQ)

Reactivity: Human, Mouse, Rat, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This 14-3-3 theta antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-14-3-3 theta Antibody

Purification: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human YWHAQ.

Application Details

Application Notes: YWHAQ antibody can be used for detection of YWHAQ by ELISA at 1:312500. YWHAQ antibody can be used for detection of YWHAQ by western blot at 1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store YWHAQ antibody at -20 °C.

Target Details for 14-3-3 theta

Target: 14-3-3 theta (YWHAQ)

Alternative Name: YWHAQ

Background: YWHAQ belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99 % identical to the mouse and rat orthologs. This gene is upregulated in patients with amyotrophic lateral sclerosis.This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99 % identical to the mouse and rat orthologs. This gene is upregulated in patients with amyotrophic lateral sclerosis. It contains in its 5' UTR a 6 bp tandem repeat sequence which is polymorphic, however, there is no correlation between the repeat number and the disease.

Molecular Weight: 28 kDa

Gene ID: 10971

NCBI Accession: NP_006817

UniProt: P27348

Pathways: Apoptosis, Myometrial Relaxation and Contraction