TNNI3 antibody (truncated)

Catalog No. ABIN2464041

The Mouse Monoclonal anti-TNNI3 antibody (Clone 3A10A12) (ABIN2464041) specifically detects TNNI3 in WB, IHC and ELISA. The antibody is reactive with Human samples.

Quick Overview for TNNI3 antibody (truncated) (ABIN2464041)

Target: TNNI3 (Cardiac Troponin I (TNNI3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TNNI3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Clone: 3A10A12

Product Details anti-TNNI3 Antibody

Binding Specificity: truncated

Immunogen: Ni-NTA purified truncated recombinant cTnI expressed in E. Coli strain BL21 (DE3).

Isotype: IgG1

Application Details

Application Notes: Western Blot:Dilution 1:500 - 1:2,000
IHC(P):Dilution 1:500 - 2,000
IHC(F):Dilution 1:500 - 2,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Ascitic fluid containing 0.03 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Storage: -20 °C

Storage Comment: cTnI monoclonal antibody can be stored at -20 °C, stable for one year.

Target Details for TNNI3

Target: TNNI3 (Cardiac Troponin I (TNNI3))

Alternative Name: cTnI

Background: CTnI has an apparent molecular weight of 22.5 kDa. cTnI is a candidate marker with acceptable sensitivity and specificity for AMI and other cardiac diseases. Troponin, a molecule that binds to the thin filament (actin) of striated muscle fibers, acts with intracellular calcium to control the interaction of the thin filament with the thick filament (myosin), thus regulating muscle contraction. Troponin I prevents muscle contraction in the absence of calcium, which has two skeletal muscle isoforms with considerable amino acid sequence homology. cTnI contains an additional N-terminal sequence and is highly specific for myocardium.

Gene ID: 7137

UniProt: P19429