HPRT1 antibody (truncated)

Catalog No. ABIN2464064

The Mouse Monoclonal anti-HPRT1 antibody has been validated for WB and ELISA. It is suitable to detect HPRT1 in samples from Human.

Quick Overview for HPRT1 antibody (truncated) (ABIN2464064)

Target: HPRT1 (Hypoxanthine phosphoribosyltransferase 1 (HPRT1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This HPRT1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Clone: 1F8D11

Product Details anti-HPRT1 Antibody

Binding Specificity: truncated

Immunogen: Ni-NTA purified truncated recombinant HPRT expressed in E. Coli strain BL21 (DE3).

Isotype: IgG2b

Application Details

Application Notes: Western Blot:Dilution 1:500 - 1:2,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.

Restrictions: For Research Use only

Handling

Format: Liquid

Handling Advice: As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Storage: -20 °C

Storage Comment: HPRT monoclonal antibody can be stored at -20 °C, stable for one year.

Target Details for HPRT1

Target: HPRT1 (Hypoxanthine phosphoribosyltransferase 1 (HPRT1))

Alternative Name: HPRT

Background: The HPRT1 gene provides instructions for making an enzyme called hypoxanthine phosphoribosyltransferase 1. This enzyme allows cells to recycle purines, some of the building blocks of DNA and its chemical cousin RNA.The enzyme hypoxanthine-guanine phosphoribosyltrasferase (E.C.2.4.2.8., HPRT) plays a crucial role in uric acid synthesis and purine metabolism.This enzyme catalyzes the conversion of hypoxanthine and guanine to inosine monophosphate (IMP) and guanosine monophosphate (GMP), respectively, and uses phosphoribosylpyrophosphate (PRPP) as a cosubstrate and as a source of energy . This pathway is also known as the purine salvage pathway because it allows cells to reuse purine compounds to build DNA and RNA.

Gene ID: 3251

UniProt: P00492

Pathways: Ribonucleoside Biosynthetic Process