RAB25 antibody

Catalog No. ABIN2464096

This Mouse Monoclonal antibody specifically detects RAB25 in ELISA, IHC and WB. It exhibits reactivity toward Human.

Quick Overview for RAB25 antibody (ABIN2464096)

Target: RAB25 (RAB25, Member RAS Oncogene Family (RAB25))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This RAB25 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Clone: 3F12F3

Product Details anti-RAB25 Antibody

Purification: Antibody is purified by protein A affinity chromatography and resuspended in PBS with 0.2%BSA, 0.09% sodium azide.

Immunogen: Ni-NTA purified recombinant Rab25 expressed in E. Coli strain BL21 (DE3).

Isotype: IgG2a

Application Details

Application Notes: Western Blot:1:500 - 1:2,000
IHC(P):1:500 - 1:2,000
IHC(F):1:500 - 1:2,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.

Restrictions: For Research Use only

Handling

Buffer: Purified antibody in PBS containing 0.03 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Storage: -20 °C

Storage Comment: Rab25 monoclonal antibody can be stored at -20 °C, stable for one year.

Target Details for RAB25

Target: RAB25 (RAB25, Member RAS Oncogene Family (RAB25))

Alternative Name: Rab25

Background: Members of the Ras-related superfamily of GTP binding proteins, which includes Ras, Rho, Rab and ARF subfamilies, exhibit 30-50 % similarity with Ras p21. Rab proteins play an important role for either in endocytosis or in biosynthetic protein transport. The possibility that Rab proteins might also direct the exocytosis from secretory vesicles to the plasma membrane is supported by the observation that in yeast, the SEC4 protein, which is 40 % similar to Rab proteins, is associated with secretory vesicles. Rab proteins located on the cytoplasmic face of organelles and vesicles, rab proteins are involved in intracellular membrane fusion reactions. Rab25 was cloned from a gastric parietal cell cDNA library and is expressed in epithelial tissues such as the gastrointestinal mucosae, kidney, and lung, which encoded a protein of 28 kDa.

Gene ID: 57111

UniProt: P57735