FOXI3 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
FOXI3 antibody was raised against a 14 amino acid synthetic peptide near the internal region of FOXI3.
FOXI3
Reactivity: Human
WB, ELISA, FACS
Host: Rabbit
Polyclonal
FITC
Application Notes
ELISA: Antibody detection limit dilution 1:4000. Western Blot: Approximately 50 kDa band in human skin lysates after 1 μg/mL antibody staining . The 50 kDa band was successfully blocked by incubation with the immunizing peptide. IHC: In paraffin embedded Human Bronchus shows strong nuclear staining in the basal cells of the resporatory epithelium. Recommended concentration, 5-10μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.