DEFA1/3 (Internal Region) antibody
Reactivity: Human
ELISA, WB
Host: Goat
Polyclonal
unconjugated
-
- Target
- DEFA1/3
- Binding Specificity
- Internal Region
- Reactivity
- Human
-
Host
- Goat
-
Clonality
- Polyclonal
-
Conjugate
- Un-conjugated
- Application
- ELISA, Western Blotting (WB)
- Specificity
- This antibody is expected to equally recognize DEFA1 precursor and DEFA3 precursor.
- Purification
- DEFA1/3 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Immunogen
- DEFA1/3 antibody was raised against a 14 amino acid synthetic peptide near the internal region of DEFA1/3.
-
-
- Application Notes
- ELISA: Antibody detection limit dilution 1:128000 Western Blot: Approximately 10 kDa band observed in human spleen lysates (calculated MW of 10.2 kDa according to NP_004075.1). Recommended concentration: 1-3 μg/mL. an additional band of unknown identity was also consistently observed at 20 kDa . This band was successfully blocked by incubation with the immunizing peptide Immunohistochemistry: In paraffin embedded thymus shows staining of selected cells. Recommended concentration, 4-6 μg/mL.
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 500 μg/mL
- Buffer
- Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Minimize freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Aliquot and store at -20 °C.
-
-
You are here:
