Prion Protein antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Prion Protein antibody was raised against an 11 amino acid synthetic peptide near the internal region of Prion Protein.
PRNP
Reactivity: Human
WB
Host: Mouse
Monoclonal
EM-20
unconjugated
Application Notes
ELISA: Antibody detection limit dilution 1:64,000. Western Blot: Approximately 25 kDa band observed in human brain lysates (calculated MW of 27.7 kDa according to NP_000302.1 and NP_898902.1). Recommended concentration: 0.3-1 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.