HTATSF1 antibody (Internal Region)

Catalog No. ABIN2465832

The Goat Polyclonal anti-HTATSF1 antibody (ABIN2465832) specifically detects HTATSF1 in WB and ELISA. The antibody is reactive with Human samples.

Quick Overview for HTATSF1 antibody (Internal Region) (ABIN2465832)

Target: HTATSF1 (HIV-1 Tat Specific Factor 1 (HTATSF1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This HTATSF1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-HTATSF1 Antibody

Binding Specificity: Internal Region

Purification: HTATSF1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: HTATSF1 antibody was raised against a 15 amino acid synthetic peptide near the internal region (near the N-Terminus) of HTATSF1.

Application Details

Application Notes: ELISA: Antibody detection limit dilution 1:16,000. Western Blot: Approximately 140 kDa band observed in lysates of Daudi lysate (calculated MW of 85.9 kDa according to NP_055315.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Zhou and Sharp, Science. 1996 Oct 25,274 (5287) :605-10., PMID: 8849451). Recommended concentration: 1-3 μg/mL. an additional band of unknown identity was also consistently observed at 20 kDa . This band was successfully blocked by incubation with the immunizing peptide.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20 °C.

Target Details for HTATSF1

Target: HTATSF1 (HIV-1 Tat Specific Factor 1 (HTATSF1))

Alternative Name: HTATSF1

Gene ID: 27336

NCBI Accession: NP_055315

UniProt: O43719