KLF4 antibody (N-Term)

Catalog No. ABIN2465877

The Goat Polyclonal anti-KLF4 antibody (ABIN2465877) specifically detects KLF4 in WB and ELISA. The antibody is reactive with Human samples.

Quick Overview for KLF4 antibody (N-Term) (ABIN2465877)

Target: KLF4 (Kruppel-Like Factor 4 (Gut) (KLF4))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This KLF4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-KLF4 Antibody

Binding Specificity: N-Term

Purification: KLF4 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: KLF4 antibody was raised against a 12 amino acid synthetic peptide near the N-Terminus of KLF4.

Application Details

Application Notes: ELISA: Antibody detection limit dilution 1:64000. Western Blot: Experiments gave strong bands at 75-90 kDa and a faint 50 kDa band in human duodenal lysate after 0.1 μg/mL antibody staining. These bands correspond to earlier findings in literature (Piccinni et al, J. Biol. Chem., Vol. 279 (10), 9103-9114). All these bands were successfully blocked by incubation with the immunizing peptide. Recommended concentration: 0.1-0.3 μg/mL.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20 °C.

Target Details for KLF4

Target: KLF4 (Kruppel-Like Factor 4 (Gut) (KLF4))

Alternative Name: KLF4

Gene ID: 9314

NCBI Accession: NP_004226

UniProt: O43474

Pathways: Peptide Hormone Metabolism, Stem Cell Maintenance