SIRT4 antibody (C-Term)
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- Target See all SIRT4 Antibodies
- SIRT4 (Sirtuin 4 (SIRT4))
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Binding Specificity
- C-Term
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Reactivity
- Human, Rat
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This SIRT4 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), ELISA
- Purification
- SIRT4 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Immunogen
- SIRT4 antibody was raised against a 13 amino acid synthetic peptide near the C-Terminus of SIRT4.
- Top Product
- Discover our top product SIRT4 Primary Antibody
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- Application Notes
- ELISA: Antibody detection limit dilution 1:32000. Western Blot: Approximately 38 kDa band observed in human kidney and U937 lysates (calculated MW of 35.2 kDa according to NP_036372.1). In transfected HEK293 transiently expressing SIRT4 a band of approximately 38 kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 1-3 μg/mL. Immunohistochemistry: In paraffin embedded skeletal muscle shows spotty staining of striated fibres in longitudinal section. Recommended concentration, 2-3 μg/mL.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 500 μg/mL
- Buffer
- Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Minimize freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Aliquot and store at -20 °C.
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- Target
- SIRT4 (Sirtuin 4 (SIRT4))
- Alternative Name
- SIRT4 (SIRT4 Products)
- Gene ID
- 23409
- NCBI Accession
- NP_036372
- UniProt
- Q9Y6E7
- Pathways
- Negative Regulation of Hormone Secretion
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