SIRT4 antibody (C-Term)

Catalog No. ABIN2466271

This Goat Polyclonal antibody specifically detects SIRT4 in WB, IHC and ELISA. It exhibits reactivity toward Human and Rat.

Quick Overview for SIRT4 antibody (C-Term) (ABIN2466271)

Target: SIRT4 (Sirtuin 4 (SIRT4))

Reactivity: Human, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This SIRT4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-SIRT4 Antibody

Binding Specificity: C-Term

Purification: SIRT4 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: SIRT4 antibody was raised against a 13 amino acid synthetic peptide near the C-Terminus of SIRT4.

Application Details

Application Notes: ELISA: Antibody detection limit dilution 1:32000. Western Blot: Approximately 38 kDa band observed in human kidney and U937 lysates (calculated MW of 35.2 kDa according to NP_036372.1). In transfected HEK293 transiently expressing SIRT4 a band of approximately 38 kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 1-3 μg/mL. Immunohistochemistry: In paraffin embedded skeletal muscle shows spotty staining of striated fibres in longitudinal section. Recommended concentration, 2-3 μg/mL.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20 °C.

Target Details for SIRT4

Target: SIRT4 (Sirtuin 4 (SIRT4))

Alternative Name: SIRT4

Gene ID: 23409

NCBI Accession: NP_036372

UniProt: Q9Y6E7

Pathways: Negative Regulation of Hormone Secretion