PIKE (Internal Region) antibody
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- Target
- PIKE
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Binding Specificity
- Internal Region
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Reactivity
- Human
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- Un-conjugated
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Application
- Western Blotting (WB), ELISA
- Specificity
- This antibody is expected to recognize isoform PIKE-S (NP_055585.1).
- Purification
- PIKE antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Immunogen
- PIKE antibody was raised against a 15 amino acid synthetic peptide near the internal region of PIKE.
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- Application Notes
- ELISA: Antibody detection limit dilution 1:8000. Western Blot: Approximately 100 kDa band observed in human brain (amygdala and cerebral cortex) lysates (calculated MW of 90.5 kDa according to NP_055585.1). Recommended concentration: 0.3-1 μg/mL.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 500 μg/mL
- Buffer
- Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Minimize freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Aliquot and store at -20 °C.
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