CRTC1 antibody (AA 19-34)

Catalog No. ABIN2470217

The Rabbit Polyclonal anti-CRTC1 antibody has been validated for WB, ELISA, IHC and IF. It is suitable to detect CRTC1 in samples from Human, Mouse, Rat, Cow, Zebrafish (Danio rerio) and Pufferfish.

Quick Overview for CRTC1 antibody (AA 19-34) (ABIN2470217)

Target: CRTC1 (CREB Regulated Transcription Coactivator 1 (CRTC1))

Reactivity: Human, Mouse, Rat, Cow, Zebrafish (Danio rerio), Pufferfish

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CRTC1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-CRTC1 Antibody

Binding Specificity: AA 19-34

Purification: Immunoaffinity Chromatography

Immunogen: CRTC1 antibody was raised against amino acids 19 - 34 of CRTC1 (Human).

Application Details

Application Notes: CRTC1 antibody can be used in ELISA starting at 1:14000 - 1:80000, Western Blot starting at 1:100 - 1:8000, immunohistochemistry starting at 5 μg/mL, and immunofluorescence.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: As with all antibodies avoid freeze/thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: Store CRTC1 antibody at 4 °C or -20 °C.

Target Details for CRTC1

Target: CRTC1 (CREB Regulated Transcription Coactivator 1 (CRTC1))

Alternative Name: CRTC1

Background: MECT1 (also known as MucoEpidermoid Carcinoma Translocated 1, Transducer of regulated cAMP response element-binding protein 1, TORC1, and Transducer of CREB protein 1) is a nuclear protein that functions as a transcriptional coactivator for CREB1, which activates transcription through both consensus and variant cAMP response element (CRE) sites. MECT1does not appear to modulate CREB1 DNA-binding activity but enhances the interaction of CREB1 with TAF4/TAFII-130. MECT1 translocates with MAML2 (MasterMind-Like Protein 2) to yield a fusion oncogene: t(11,19) (q21,p13). This translocation occurs in mucoepidermoid carcinomas, benign Warthin tumors and clear cell hidradenomas. The novel fusion product that results disrupts the Notch signaling pathway. The fusion protein consists of the N-terminus of MECT1 joined to the C-terminus of MAML2. The reciprocal fusion protein consisting of the N-terminus of MAML2 joined to the C-terminus of MECT1 has been detected in a small number of mucoepidermoid carcinomas. Multiple isoforms have been reported for the MECT1 protein.

Gene ID: 23373

UniProt: Q6UUV9