Cullin 3 antibody (N-Term)

Catalog No. ABIN2470220

The Rabbit Polyclonal anti-Cullin 3 antibody has been validated for WB, IHC, ELISA and IP. It is suitable to detect Cullin 3 in samples from Human.

Quick Overview for Cullin 3 antibody (N-Term) (ABIN2470220)

Target: Cullin 3 (CUL3)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cullin 3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP)

Product Details anti-Cullin 3 Antibody

Binding Specificity: N-Term

Purification: Delipidation and Defibrination

Immunogen: Cullin 3 antibody was raised against amino acids 1-12 from the N-Terminus of Cullin 3 (Human).

Application Details

Application Notes: Cullin 3 antibody can be used in ELISA starting at 1:1000, and immunohistochemistry starting at 10 μg/mL.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.01 % Sodium azide

Handling Advice: As with all antibodies avoid freeze/thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: Store Cullin 3 antibody at 4 °C or -20 °C.

Target Details for Cullin 3

Target: Cullin 3 (CUL3)

Alternative Name: Cullin 3

Background: Cullins assemble a potentially large number of ubiquitin ligases by binding to the RING protein ROC1 to catalyse polyubiquitination, as well as binding to various specificity factors to recruit substrates. Cullin 3 is an essential component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, cul3 serves as a rigid scaffold that organizes the SKP1-F-box protein and RBX1 subunits. Cul3 may also contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. Unlike Cul1 and Cul2, Cul3 seems not to be a part of the SCF complex consisting of CUL1, RBX1, SKP1 and SKP2. Cul3 also interacts with RNF7 and is part of a complex with TIP120A/CAND1, Cyclin E and RBX1.

Gene ID: 8452

UniProt: Q13618

Pathways: M Phase