NOTCH2 antibody (N-Term)

Catalog No. ABIN2470258

This Rabbit Polyclonal antibody specifically detects NOTCH2 in WB, ELISA, IHC and IF. It exhibits reactivity toward Human, Dog and Chimpanzee.

Quick Overview for NOTCH2 antibody (N-Term) (ABIN2470258)

Target: NOTCH2 (Notch 2 (NOTCH2))

Reactivity: Human, Dog, Chimpanzee

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NOTCH2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-NOTCH2 Antibody

Binding Specificity: N-Term

Purification: Sterile Filtration

Immunogen: NOTCH2 antibody was raised against amino acid residues 2396 - 2409 of NOTCH2. A residue of cysteine was added to the amino terminal end to facilitate coupling (Human).

Application Details

Application Notes: NOTCH2 antibody can be used in Western Blot starting at 0.5 - 4 μg/mL, and immunohistochemistry starting at 5 μg/mL.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: As with all antibodies avoid freeze/thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: Store NOTCH2 antibody at 4 °C or -20 °C.

Target Details for NOTCH2

Target: NOTCH2 (Notch 2 (NOTCH2))

Alternative Name: NOTCH2

Background: Notch is synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase (S1 cleavage) in the trans-golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin-dependent gamma-secretase (S3 cleavage) to release the intracellular domain (NICD) from the membrane. Notch functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBP-J kappa and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs.

Gene ID: 4853

UniProt: Q04721

Pathways: Notch Signaling, Stem Cell Maintenance