AHSA1 antibody (Biotin)

Catalog No. ABIN2481472

This Rat Monoclonal antibody specifically detects AHSA1 in WB, ELISA, IHC, IP, IF and ICC. It exhibits reactivity toward Mouse.

Quick Overview for AHSA1 antibody (Biotin) (ABIN2481472)

Target: AHSA1 (Activator of HSP90 ATPase Activity 1 (AHSA1))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This AHSA1 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: 25F2-D9

Product Details anti-AHSA1 Antibody (Biotin)

Specificity: Detects ~38 kDa. Can run up to 45 kDa on SDS Page.

Cross-Reactivity: Human, Mouse, Rat

Purification: Protein G Purified

Immunogen: Recombinant Full Length Mouse Aha1 Protein

Isotype: IgG2a kappa

Application Details

Application Notes:

• WB (1:1000)
• IHC (1:100)
• ICC/IF (1:1000)
• IP (1:1000)
• optimal dilutions for assays should be determined by the user.

Comment:

1 μg/ml of ABIN2481472 was sufficient for detection of Aha1 in 10 μg of rat tissue lysate by colorimetric immunoblot analysis using Goat anti-rat IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.2 mg/mL

Buffer: 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, Storage buffer may change when conjugated

Storage: 4 °C

Storage Comment: Conjugated antibodies should be stored at 4°C

Target Details for AHSA1

Target: AHSA1 (Activator of HSP90 ATPase Activity 1 (AHSA1))

Alternative Name: AHA1

Background: Aha1 is a member of the HSP90 cochaperone family, and is thought to stimulate HSP90 ATPase activity by competing with p23 and other co-chaperones for HSP90 binding (1, 2). It may affect a step in the endoplasmic reticulum to Golgi trafficking. Aha1 also interacts with HSPCA/HSP90 and with the cytoplasmic tail of the vesicular stomatistis virus glycoproteins (VSV G) (3). Aha1 is expressed in numerous tissues, including the brain, heart, skeletal muscle, and kidney, and at low levels, the liver and placenta. Aha1 might be a potential therapeutic strategy to increase sensitivity to HSP inhibitors (4).

Gene ID: 217737

NCBI Accession: NP_666148

UniProt: Q8BK64