DNAJB1 antibody (FITC)

Catalog No. ABIN2481541

This Mouse Monoclonal antibody specifically detects DNAJB1 in WB, ELISA and IP. It exhibits reactivity toward Saccharomyces cerevisiae.

Quick Overview for DNAJB1 antibody (FITC) (ABIN2481541)

Target: DNAJB1 (DnaJ (Hsp40) Homolog, Subfamily B, Member 1 (DNAJB1))

Reactivity: Saccharomyces cerevisiae

Host: Mouse

Clonality: Monoclonal

Conjugate: This DNAJB1 antibody is conjugated to FITC

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Clone: 1G10-H8

Product Details anti-DNAJB1 Antibody (FITC)

Specificity: Detects ~40 kDa. Yeast specific product. Does not cross react with Human, Mouse or Rat.

Cross-Reactivity: Saccharomyces cerevisiae

Purification: Protein G Purified

Immunogen: Full length protein yeast HSP40 (YDJ1)

Isotype: IgG1 kappa

Application Details

Application Notes:

• WB (1:2000)
• optimal dilutions for assays should be determined by the user.

Comment:

0.5 μg/ml of ABIN2481541 was sufficient for detection of 50 ng YDJ1 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Conjugated antibodies should be stored at 4°C

Target Details for DNAJB1

Target: DNAJB1 (DnaJ (Hsp40) Homolog, Subfamily B, Member 1 (DNAJB1))

Alternative Name: HSP40

Background: Human HSP40/DnaJ proteins comprise a large protein family, members of which feature the J domain (named after the bacterial DnaJ protein) (1). The J-domain spans the first 75 N-terminal amino acids and is separated from the C-terminal by a glycine/phenylalanine-rich domain (2). There are two main types of HSP40, type 1 DNAJ proteins including HDJ2 and yeast YdjI, type II includes yeast Sis1 and human Hdj1. Whereas type I possesses a zinc finger domain which helps in the function of protein folding. (3, 4), type II does not. Members of the HSP40/DnaJ family play diverse roles in many cellular processes, such as folding, translocation, degradation and assembly of multi-protein complexes. HSP40 stimulates the ATPase activity of HSP70 which in turn causes conformational changes of the unfolded proteins (5, 6). The HSP40-HSP70-unfolded protein complex further binds to co-chaperones Hip, Hop and HSP90 which leads to protein folding, or components of protein degradation machinery CHIP and BAG-1 (7).

Gene ID: 855661

NCBI Accession: NP_014335

UniProt: P25491