CRYAA antibody (PerCP)

Catalog No. ABIN2481848

The Mouse Monoclonal anti-CRYAA antibody has been validated for WB, ELISA, IF and ICC. It is suitable to detect CRYAA in samples from Human.

Quick Overview for CRYAA antibody (PerCP) (ABIN2481848)

Target: CRYAA (Crystallin, alpha A (CRYAA))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CRYAA antibody is conjugated to PerCP

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: 1H3-B8

Product Details anti-CRYAA Antibody (PerCP)

Specificity: Detects ~20 kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.

Cross-Reactivity: Cow, Human, Mouse, Rat

Purification: Protein G Purified

Immunogen: Native Alpha Crystallin

Isotype: IgG1

Application Details

Application Notes:

• WB (1:2000)
• ICC/IF (1:100)
• optimal dilutions for assays should be determined by the user.

Comment:

0.5 μg/ml was sufficient for detection of 100 ng purified alphaA crystalline by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.2, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Conjugated antibodies should be stored at 4°C

Target Details for CRYAA

Target: CRYAA (Crystallin, alpha A (CRYAA))

Alternative Name: Alpha A Crystallin

Background: The alpha-crystallins are major water-soluble lens structural proteins of the vertebrate eye that are related to the small heat shock protein family. The alpha-crystallins possess structural and functional similarities with HSP25 and HSP27 (1). Mammalian lens cystallins are divided into alpha, beta and gamma families. Alpha and beta families are further divided into acidic and basic groups (Alpha-A and Alpha-B respectively). In the lens, alpha-crystallin primarily functions to maintain proper refractive index, however it can also function as a molecular chaperone that binds to the denatured proteins, keeping them in solution and thereby maintaining the translucency of the lens. When cellular stress occurs, alpha-crystallin enters its' phosphorylated state and may serve a structural control function and play a role in protein maintenance (2). In addition to their interaction with proteins, alpha-crystallins also interact with native molecules such as membrane proteins, Golgi matrix protein, structural proteins, nuclear proteins and DNA (3, 4, 5, 6, and 7). Two other functions are an autokinase activity and participation in the intracellular architecture, and it has also been proven that both alpha-A and B prevent apoptosis by inhibiting caspases (8).

Gene ID: 1409

NCBI Accession: NP_000385

UniProt: P02489

Pathways: M Phase