HSPA4 antibody (Biotin)

Catalog No. ABIN2481914

The Rabbit Polyclonal anti-HSPA4 antibody has been validated for WB, IHC, IF and ICC. It is suitable to detect HSPA4 in samples from Hamster.

Quick Overview for HSPA4 antibody (Biotin) (ABIN2481914)

Target: HSPA4 (Heat Shock 70kDa Protein 4 (HSPA4))

Reactivity: Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HSPA4 antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-HSPA4 Antibody (Biotin)

Specificity: Detects ~110 kDa.

Cross-Reactivity: Cow, Hamster, Human, Monkey, Mouse, Rat, Saccharomyces cerevisiae, Shark, Sheep

Purification: Protein A Purified

Immunogen: Synthetic peptide derived from the sequence of hamster HSP110, sequence identical to human and mouse

Application Details

Application Notes:

• WB (1:1000)
• IHC (1:100)
• optimal dilutions for assays should be determined by the user.

Comment:

1 μg/ml of ABIN2481914 was sufficient for detection of HSP110 in 10 μg of human cell line mixed lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.2, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Conjugated antibodies should be stored at 4°C

Target Details for HSPA4

Target: HSPA4 (Heat Shock 70kDa Protein 4 (HSPA4))

Alternative Name: HSP110

Background: HSP110 belongs to a family of large stress proteins known as the HSP110/SSE Family. The proteins in this family are the most distantly known relatives of the well studied HSP70 family. They share 30-33 % amino acid identity, mostly in the conserved ATP-binding domain (1). HSP110 cooperates with HSP70 in protein folding in the eukaryotic cytosol (2). In mammals, HSP110 is constitutively expressed, but exhibits particularly high levels in the brain. Both HSP70 and HSP110 are elevated after cerebral ischemia. Recent studies demonstrate that the protective effects of HSP110 deficiency in cerebral ischemia may partly be mediated by an increase in the chaperone activity of HSP70 (3). Studies also suggest that HSP110 can be used in heat shock protein-based cancer immunotherapy (4).

Gene ID: 10808

NCBI Accession: NP_006635

UniProt: Q92599