DUX4 antibody (C-Term) (Atto 390)

Catalog No. ABIN2482286

This anti-DUX4 antibody is a Mouse Monoclonal antibody detecting DUX4 in WB, IHC, IF and ICC. Suitable for Human.

Quick Overview for DUX4 antibody (C-Term) (Atto 390) (ABIN2482286)

Target: DUX4 (Double Homeobox 4 (DUX4))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This DUX4 antibody is conjugated to Atto 390

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: P2B1

Product Details anti-DUX4 Antibody (Atto 390)

Binding Specificity: C-Term

Specificity: Detects ~45 kDa. No cross-reactivity with DUX4c.

Cross-Reactivity: Human, Mouse

Purification: Protein G Purified

Immunogen: C-terminal 76 amino acids of DUX4 with glutathione-s-transferase (gst) tag

Isotype: IgG1

Application Details

Application Notes:

• WB (1:1000)
• ICC/IF (1:1000)
• optimal dilutions for assays should be determined by the user.

Comment:

1 μg/ml of ABIN2482286 was sufficient for detection of DUX4 in 20 μg of HeLa cell lysate by ECL immunoblot analysis using goat anti-mouse IgG: HRP as teh secondary.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Conjugated antibodies should be stored at 4°C

Target Details for DUX4

Target: DUX4 (Double Homeobox 4 (DUX4))

Alternative Name: DUX4

Background: DUX4, or double homeobox4, is a human protein that is a transcriptional activator of paired-like homeodomain transcription factor 1 (1). Clinically it is a facioscapulohumeral muscular dystrophy candidate gene that appears to have a toxic gain of function (2-4). In FSHD individuals, the expression of the full-length DUX4 transcript is not completely suppressed in skeletal muscle and possibly other differentiated tissues (5).

Gene ID: 22947

NCBI Accession: NP_149418

UniProt: F5GZ66