Piccolo antibody (Atto 488)

Catalog No. ABIN2482389

This Rabbit Polyclonal antibody specifically detects Piccolo in WB and IHC. It exhibits reactivity toward Human.

Quick Overview for Piccolo antibody (Atto 488) (ABIN2482389)

Target: Piccolo (PIC)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Piccolo antibody is conjugated to Atto 488

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Piccolo Antibody (Atto 488)

Specificity: Detects ~550 kDa. No cross-reactivity to Bassoon.

Cross-Reactivity: Human, Mouse, Rat

Purification: Protein A Purified

Immunogen: Recombinant Full length Protein, BC001304.1

Application Details

Application Notes:

• WB (1:1000)
• IHC (1:400)
• optimal dilutions for assays should be determined by the user.

Comment:

1 μg/ml of ABIN2482389 was sufficient for detection of Piccolo in 10 μg of rat brain tissue lysate by colorimetric immunoblot analysis using goat ant rabbit IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Conjugated antibodies should be stored at 4°C

Target Details for Piccolo

Target: Piccolo (PIC)

Alternative Name: Piccolo

Background: Piccolo, also referred to as Aczonin, is a large protein which consists of an N-terminal Zn2+ finger, several piccolo-bassoon homology domains and C-terminal PDZ and C2 domains (1, 2). In general it is found together with bassoon, a related huge multi-domain protein of the CAZ (cytoskeletal matric assembled at active zones) (3). Piccolo is a scaffolding protein for proteins involved in endo- and exocytosis of synaptic vesicles. Piccolo has also been shown to interfere with clathrin medicated endocytosis by binding to the F-actin and dynamin binding protein Abp1 (2).

Gene ID: 27445

NCBI Accession: NP_055325

UniProt: Q9Y6V0