HCN4 antibody (AA 1019-1198) (FITC)

Catalog No. ABIN2482537

The Mouse Monoclonal anti-HCN4 antibody is suitable to detect HCN4 in samples from Rat. It has been validated for WB, IHC, IF, ICC, FACS and AA.

Quick Overview for HCN4 antibody (AA 1019-1198) (FITC) (ABIN2482537)

Target: HCN4 (Hyperpolarization Activated Cyclic Nucleotide-Gated Potassium Channel 4 (HCN4))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This HCN4 antibody is conjugated to FITC

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS), Antibody Array (AA)

Clone: N114-10 (Formerly S114-10)

Product Details anti-HCN4 Antibody (FITC)

Binding Specificity: AA 1019-1198

Specificity: Detects ~130 kDa. No cross-reactivity against other HCNs.

Cross-Reactivity: Human, Mouse, Rabbit, Rat

Purification: Protein G Purified

Immunogen: Fusion protein amino acids 1019-1198 (c-terminus) of rat HCN4

Isotype: IgG1

Application Details

Application Notes:

• WB (1:1000)
• IHC (1:1000)
• ICC/IF (1:100)
• optimal dilutions for assays should be determined by the user.

Comment:

1 μg/ml of ABIN2482537 was sufficient for detection of HCN4 in 10 μg of COS cell lysate transiently transfected with HCN4 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Conjugated antibodies should be stored at 4°C

Target Details for HCN4

Target: HCN4 (Hyperpolarization Activated Cyclic Nucleotide-Gated Potassium Channel 4 (HCN4))

Alternative Name: HCN4

Background: Hyperpolarization-activated cation channels of the HCN gene family contribute to spontaneous rhythmic activity in both the heart and brain (1).

Gene ID: 59266

NCBI Accession: NP_067690

UniProt: Q9JKA7