ABCC8 antibody (AA 1548-1582) (Atto 488)

Catalog No. ABIN2482986

This Mouse Monoclonal antibody specifically detects ABCC8 in WB, IHC, ICC and IF. It exhibits reactivity toward Rat.

Quick Overview for ABCC8 antibody (AA 1548-1582) (Atto 488) (ABIN2482986)

Target: ABCC8 (ATP-Binding Cassette, Sub-Family C (CFTR/MRP), Member 8 (ABCC8))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This ABCC8 antibody is conjugated to Atto 488

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)

Clone: S289-16

Product Details anti-ABCC8 Antibody (Atto 488)

Binding Specificity: AA 1548-1582

Specificity: Detects ~160 kDa. Does not cross-react with SUR2B.

Cross-Reactivity: Hamster, Human, Mouse, Rat

Purification: Protein G Purified

Immunogen: Fusion protein amino acids 1548-1582 (cytoplasmic C-terminus) of rat SUR1

Isotype: IgG1

Application Details

Application Notes:

• WB (1:1000)
• IHC (1:1000)
• ICC/IF (1:100)
• optimal dilutions for assays should be determined by the user.

Comment:

1 μg/ml of ABIN2482986 was sufficient for detection of SUR1 in 20 μg of mouse brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Conjugated antibodies should be stored at 4°C

Target Details for ABCC8

Target: ABCC8 (ATP-Binding Cassette, Sub-Family C (CFTR/MRP), Member 8 (ABCC8))

Alternative Name: SUR1

Background: Sulfonylurea receptors (SUR) are membrane proteins which are the molecular targets of the sulfonylurea class of anti-diabetic drugs whose mechanism of action is to promote insulin release from pancreatic beta cells. More specifically, SUR proteins are subunits of the inward-rectifier potassium ion channels Kir6.x (6.1 and 6.2) (1). The association of four Kir6.x and four SUR subunits form an ion conducting channel commonly referred to as the KATP channel. The primary function of the sulfonylurea receptor is to sense intracellular levels of the nucleotides ATP and ADP and in response facilitate the open or closing its associated Kir6.x potassium channel. Hence the KATP channel monitors the energy balance within the cell (2).

Gene ID: 25559

NCBI Accession: NP_037171

UniProt: Q09429

Pathways: Negative Regulation of Hormone Secretion