GABRA1 antibody (AA 370-433) (Atto 390)

Catalog No. ABIN2483478

This Mouse Monoclonal antibody specifically detects GABRA1 in WB, IHC, ICC, IF and AA. It exhibits reactivity toward Mouse.

Quick Overview for GABRA1 antibody (AA 370-433) (Atto 390) (ABIN2483478)

Target: GABRA1 (gamma-aminobutyric Acid (GABA) A Receptor, alpha 1 (GABRA1))

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This GABRA1 antibody is conjugated to Atto 390

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Antibody Array (AA)

Clone: N87-25 (Formerly S87-25)

Product Details anti-GABRA1 Antibody (Atto 390)

Binding Specificity: AA 370-433

Specificity: Detects ~55 kDa. No cross-reactivity against GABA-A-R-Beta 2 or -Beta1.

Cross-Reactivity: Human, Mouse, Rat

Purification: Protein G Purified

Immunogen: Fusion protein amino acids 370-433 of mouse GABA-A-R-Beta 3

Isotype: IgG1

Application Details

Application Notes:

• WB (1:1000)
• IHC (1:1000)
• ICC/IF (1:100)
• optimal dilutions for assays should be determined by the user.

Comment:

1 μg/ml of ABIN2483478 was sufficient for detection of Beta 3 GABA receptor in 10 μg of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Conjugated antibodies should be stored at 4°C

Target Details for GABRA1

Target: GABRA1 (gamma-aminobutyric Acid (GABA) A Receptor, alpha 1 (GABRA1))

Alternative Name: GABA A Receptor

Background: The GABA-A receptor is a member of the superfamily of fast acting ligand-gated ion channels. The individual subunits of these receptors have similar sequences and structural features (1). GABA-A receptors are the major fast inhibitory neurotransmitter gated ion channels in the brain (2).

Gene ID: 14402

NCBI Accession: NP_032097

UniProt: P63080