AMIGO1 antibody (AA 554-574) (PerCP)

Catalog No. ABIN2483815

The Mouse Monoclonal anti-AMIGO1 antibody has been validated for WB, IHC, ICC and IF. It is suitable to detect AMIGO1 in samples from Human.

Quick Overview for AMIGO1 antibody (AA 554-574) (PerCP) (ABIN2483815)

Target: AMIGO1 (Adhesion Molecule with Ig-Like Domain 1 (AMIGO1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This AMIGO1 antibody is conjugated to PerCP

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)

Clone: S86-36

Product Details anti-AMIGO1 Antibody (PerCP)

Binding Specificity: AA 554-574

Specificity: Detects ~60-80 kDa depending on maturity/glycosylation.

Cross-Reactivity: Human, Mouse, Rat

Purification: Protein G Purified

Immunogen: Fusion protein amino acids 554-574 (cytoplasmic C-terminus) of human AMIGO-1

Isotype: IgG1

Application Details

Application Notes:

• WB (1:1000)
• optimal dilutions for assays should be determined by the user.

Comment:

1 μg/ml of ABIN2483815 was sufficient for detection of AMIGO-1 in 20 μg of rat brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Conjugated antibodies should be stored at 4°C

Target Details for AMIGO1

Target: AMIGO1 (Adhesion Molecule with Ig-Like Domain 1 (AMIGO1))

Alternative Name: AMIGO1

Background: The amphoterin-induced gene and ORF (AMIGO) family of proteins consists of AMIGO1, AMIGO2 and AMIGO3. All three members are single pass type I membrane proteins that contain several leucine-rich repeats, one IgG domain and a transmembrane domain. The AMIGO proteins are specifically expressed on fiber tracts of neuronal tissues and participate in their formation. They can form complexes with each other, but can also self-bind. AMIGO1, also designated Alivin2, promotes growth and fasciculation of neurites and plays a role in myelination and fasciculation of developing neural axons. In cerebellar neurons, AMIGO2 (Alivin1) is crucial for depolarization-dependent survival. Similar to AMIGO1 and AMIGO2, AMIGO3 (Alivin3) plays a role in hemophilic and/or heterophilic cell-cell interaction and signal transduction.

Gene ID: 57463

NCBI Accession: NP_065754

UniProt: Q86WK6