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AMIGO1 antibody (AA 554-574) (Atto 594)

AMIGO1 Reactivity: Human WB, IHC, ICC, IF Host: Mouse Monoclonal S86-36 Atto 594
Catalog No. ABIN2483804
  • Target See all AMIGO1 Antibodies
    AMIGO1 (Adhesion Molecule with Ig-Like Domain 1 (AMIGO1))
    Binding Specificity
    • 15
    • 12
    • 9
    • 8
    • 5
    • 5
    • 1
    AA 554-574
    Reactivity
    • 40
    • 23
    • 8
    • 4
    • 4
    • 4
    Human
    Host
    • 41
    • 14
    Mouse
    Clonality
    • 41
    • 14
    Monoclonal
    Conjugate
    • 13
    • 6
    • 5
    • 5
    • 4
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This AMIGO1 antibody is conjugated to Atto 594
    Application
    • 50
    • 19
    • 16
    • 14
    • 13
    • 13
    • 11
    • 4
    • 3
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)
    Specificity
    Detects ~60-80 kDa depending on maturity/glycosylation.
    Cross-Reactivity
    Human, Mouse, Rat
    Purification
    Protein G Purified
    Immunogen
    Fusion protein amino acids 554-574 (cytoplasmic C-terminus) of human AMIGO-1
    Clone
    S86-36
    Isotype
    IgG1
    Top Product
    Discover our top product AMIGO1 Primary Antibody
  • Application Notes
    • WB (1:1000)
    • optimal dilutions for assays should be determined by the user.
    Comment

    1 μg/ml of ABIN2483804 was sufficient for detection of AMIGO-1 in 20 μg of rat brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
    Storage Comment
    Conjugated antibodies should be stored at 4°C
  • Target
    AMIGO1 (Adhesion Molecule with Ig-Like Domain 1 (AMIGO1))
    Alternative Name
    AMIGO1 (AMIGO1 Products)
    Synonyms
    A antibody, Amigo antibody, ali2 antibody, AMIGO antibody, AMIGO-1 antibody, adhesion molecule with Ig like domain 1 antibody, Amigo1 antibody, AMIGO1 antibody
    Background
    The amphoterin-induced gene and ORF (AMIGO) family of proteins consists of AMIGO1, AMIGO2 and AMIGO3. All three members are single pass type I membrane proteins that contain several leucine-rich repeats, one IgG domain and a transmembrane domain. The AMIGO proteins are specifically expressed on fiber tracts of neuronal tissues and participate in their formation. They can form complexes with each other, but can also self-bind. AMIGO1, also designated Alivin2, promotes growth and fasciculation of neurites and plays a role in myelination and fasciculation of developing neural axons. In cerebellar neurons, AMIGO2 (Alivin1) is crucial for depolarization-dependent survival. Similar to AMIGO1 and AMIGO2, AMIGO3 (Alivin3) plays a role in hemophilic and/or heterophilic cell-cell interaction and signal transduction.
    Gene ID
    57463
    NCBI Accession
    NP_065754
    UniProt
    Q86WK6
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