SOD3 antibody (APC)

Catalog No. ABIN2484269

This anti-SOD3 antibody is a Mouse Monoclonal antibody detecting SOD3 in WB, IHC, ELISA, ICC and IF. Suitable for Human.

Quick Overview for SOD3 antibody (APC) (ABIN2484269)

Target: SOD3 (Superoxide Dismutase 3, Extracellular (SOD3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SOD3 antibody is conjugated to APC

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)

Clone: 4GG11G6

Product Details anti-SOD3 Antibody (APC)

Specificity: Detects extracellular SOD ~35 kDa.

Cross-Reactivity: Guinea Pig, Human, Mouse, Rat

Purification: Protein G Purified

Immunogen: Human extracellular SOD purified from aortas

Isotype: IgG1 kappa

Application Details

Application Notes:

• WB (1:1000)
• IHC (1:100)
• ICC/IF (1:100)
• optimal dilutions for assays should be determined by the user.

Comment:

1 μg/ml of ABIN2484269 was sufficient for detection of EC-SOD in 20 μg of human cartilage lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Conjugated antibodies should be stored at 4°C

Target Details for SOD3

Target: SOD3 (Superoxide Dismutase 3, Extracellular (SOD3))

Alternative Name: SOD3

Background: Superoxide dismutase (SOD) is an endogenously produced intracellular enzyme present in almost every cell in the body (3). It works by catalyzing the dismutation of the superoxide radical O2ˉ to O2 and H2O2, which are then metabolized to H2O and O2 by catalase and glutathione peroxidase (2, 5). In general, SODs play a major role in antioxidant defense mechanisms (4). There are three types of SOD in mammalian cells. One form (SOD1) contains Cu and Zn ions as a homodimer and exists in the cytoplasm. The two subunits of 16 kDa each are linked by two cysteines forming an intra-subunit disulphide bridge (3). The second form (SOD2) is a manganese containing enzyme and resides in the mitochondrial matrix. It is a homotetramer of 80 kDa. The third form (SOD3 or EC-SOD) is like SOD1 in that it contains Cu and Zn ions, however it is distinct in that it is a homotetramer, with a mass of 30 kDA and it exists only in the extra-cellular space (6). SOD3 can also be distinguished by its heparin-binding capacity (1).

Gene ID: 6649

NCBI Accession: NP_003093

UniProt: P08294