SERPINH1 antibody (Atto 594)

Catalog No. ABIN2484365

This anti-SERPINH1 antibody is a Mouse Monoclonal antibody detecting SERPINH1 in WB, IF and ICC. Suitable for Human.

Quick Overview for SERPINH1 antibody (Atto 594) (ABIN2484365)

Target: SERPINH1 (Serpin Family H Member 1 (SERPINH1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SERPINH1 antibody is conjugated to Atto 594

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: 1C4-1A6

Product Details anti-SERPINH1 Antibody (Atto 594)

Specificity: Detects 47 kDa.

Cross-Reactivity: Human

Purification: Protein G Purified

Immunogen: Human HSP47, full length

Isotype: IgG1 kappa

Application Details

Application Notes:

• WB (1:1000)
• ICC/IF (1:100)
• optimal dilutions for assays should be determined by the user.

Comment:

1 μg/ml of ABIN2484365 was sufficient for detection of HSP47 in 20 μg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Conjugated antibodies should be stored at 4°C

Target Details for SERPINH1

Target: SERPINH1 (Serpin Family H Member 1 (SERPINH1))

Alternative Name: HSP47

Background: HSP47 is a chaperone protein, member of the superfamily of serine proteinase inhibitors. Also known as SERPINH1, a serine proteinase inhibitor. It is a stress protein that resides in the endoplasmic reticulum, has an active role on the intracellular process of folding, assembly and secretion of pro-collagens. Recent studies have shown the association of on an increased expression of HSP47 around fibrotic lesions (1). The identification of a novel biomarker on cell therapies aimed to reduce the progression of fibrotic diseases, could be used potentially as a universal marker, since HSP47 binds a single substrate (2). Type I collagen is fundamental during the healing process after a myocardial infarction. It is critical in the position of collagen-produced cells and the assembly of collagen fibrils (3).

Gene ID: 871

NCBI Accession: NP_001193943

UniProt: P50454