CAMKII gamma antibody (Atto 390)

Catalog No. ABIN2484549

This Mouse Monoclonal antibody specifically detects CAMKII gamma in WB, IHC, ELISA, IP, IF, ICC and RIA. It exhibits reactivity toward Rat.

Quick Overview for CAMKII gamma antibody (Atto 390) (ABIN2484549)

Target: CAMKII gamma (CAMK2G) (Calcium/calmodulin-Dependent Protein Kinase II gamma (CAMK2G))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CAMKII gamma antibody is conjugated to Atto 390

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC), Radioimmunoassay (RIA)

Clone: 6G9

Product Details anti-CAMKII gamma Antibody (Atto 390)

Specificity: Detects ~50-60 kDa. Recognizes both phosphorylated and non-phosphorylated forms.

Cross-Reactivity: Cow, Human, Mouse, Rat

Purification: Protein G Purified

Immunogen: Partially purified rat CaMKII

Isotype: IgG1

Application Details

Application Notes:

• WB (1:10000)
• IHC (1:2000)
• ICC/IF (1:50)
• optimal dilutions for assays should be determined by the user.

Comment:

0.1 μg/ml was sufficient for detection of CamKII in 20 μg rat brain tissue extract by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:AP as the secondary.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Conjugated antibodies should be stored at 4°C

Target Details for CAMKII gamma

Target: CAMKII gamma (CAMK2G) (Calcium/calmodulin-Dependent Protein Kinase II gamma (CAMK2G))

Alternative Name: CaMKII

Background: CaMKII is an important member of the calcium/calmodulin-activated protein kinase family, functioning in neural synaptic stimulation and T-cell receptor signaling (1, 2). CaMKII is expressed in many different tissues but is specifically found in the neurons of the forebrain and its mRNA is found within the dendrites and the soma of the neuron. The CaMKII that is found in the neurons consist of two subunits of 52 (termed alpha genes) and 60 kDa (beta genes). CaMKII has catalytic and regulatory domains, as well as an ATP-binding domain, and a consensus phosphorylation site (3-7). The binding of Ca2+/calmodulin to its regulatory domain releases its auto inhibitory effect and activates the kinase (8). This kinase activation results in autophosphorylation at threonine 286 (8). The threonine phosphorylation state of CaMKII can be regulated through PP1/PKA. Whereas PP1 (protein phosphatase 1) dephosphorylates phospho-CaMKII at Thr286, PKA (protein kinase A) prevents this dephosphorylation (9). Autophosphorylation also enables CaMKII to attain an enhanced affinity for NMDA receptors in postsynaptic densities (10-12).

Gene ID: 12322

NCBI Accession: NP_033922

UniProt: P11798

Pathways: WNT Signaling, Interferon-gamma Pathway, Hormone Transport, Myometrial Relaxation and Contraction, Regulation of long-term Neuronal Synaptic Plasticity