SOD2 antibody (Atto 390)

Catalog No. ABIN2484754

The Rabbit Polyclonal anti-SOD2 antibody has been validated for WB, IHC, ELISA, IP, IF and ICC. It is suitable to detect SOD2 in samples from Rat.

Quick Overview for SOD2 antibody (Atto 390) (ABIN2484754)

Target: SOD2 (Superoxide Dismutase 2, Mitochondrial (SOD2))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SOD2 antibody is conjugated to Atto 390

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-SOD2 Antibody (Atto 390)

Specificity: Detects ~25 kDa.

Cross-Reactivity: Chicken, Cow, Dog, Drosophila melanogaster, Guinea Pig, Hamster, Human, Invertebrate, Monkey, Mouse, Pig, Rabbit, Rat, Sea Squirt, Sheep, Squirrel, Xenopus laevis

Purification: Protein A Purified

Immunogen: Recombinant Rat Mn SOD Protein

Application Details

Application Notes:

• WB (1:5000)
• IHC (1:100)
• ICC/IF (1:120)
• optimal dilutions for assays should be determined by the user.

Comment:

0.5 μg/ml of ABIN2484754 was sufficient for detection of Mn SOD in 20 μg of rat brain tissue extract by colorimetric immunoblot analysis using Goat anti-rabbit IgG:AP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Conjugated antibodies should be stored at 4°C

Target Details for SOD2

Target: SOD2 (Superoxide Dismutase 2, Mitochondrial (SOD2))

Alternative Name: SOD2

Background: Superoxide dismutase (SOD) is an endogenously produced intracellular enzyme present in almost every cell in the body (3). It works by catalyzing the dismutation of the superoxide radical O2ˉ to O2 and H2O2, which are then metabolized to H2O and O2 by catalase and glutathione peroxidase (2,5). In general, SODs play a major role in antioxidant defense mechanisms (4). There are two main types of SOD in mammalian cells. One form (SOD1) contains Cu and Zn ions as a homodimer and exists in the cytoplasm. The two subunits of 16 kDa each are linked by two cysteines forming an intra-subunit disulphide bridge (3). The second form (SOD2) is a manganese containing enzyme and resides in the mitochondrial matrix. It is a homotetramer of 80 kDa. The third form (SOD3 or EC-SOD) is like SOD1 in that it contains Cu and Zn ions, however it is distinct in that it is a homotetramer, with a mass of 30 kDA and it exists only in the extra-cellular space (7). SOD3 can also be distinguished by its heparin-binding capacity (1).

Gene ID: 24787

NCBI Accession: NP_058747

UniProt: P07895

Pathways: Sensory Perception of Sound, Transition Metal Ion Homeostasis, Negative Regulation of intrinsic apoptotic Signaling