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Alginate antibody (FITC)

The Mouse Monoclonal anti- antibody has been validated for IHC and WB. It is suitable to detect in samples from .
Catalog No. ABIN2484868

Quick Overview for Alginate antibody (FITC) (ABIN2484868)

Target

Alginate

Reactivity

Please inquire

Host

  • 10
Mouse

Clonality

  • 10
Monoclonal

Conjugate

  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
FITC

Application

  • 9
  • 9
  • 1
Immunohistochemistry (IHC), Western Blotting (WB)

Clone

4B10-1C5
  • Specificity

    Binds selectively to a BSA-conjugated alginate, but not to unconjugated BSA.

    Purification

    Protein G Purified

    Immunogen

    Sodium Alginate conjugated to KLH

    Isotype

    IgG1 kappa
  • Application Notes

    • WB (1:500)
    • IHC (1:200)
    • optimal dilutions for assays should be determined by the user.

    Comment

    1 μg/ml of ABIN2484868 was sufficient for detection of 150 ng of alginate-conjugated BSA by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    1 mg/mL

    Buffer

    PBS pH 7.4, 50 % glycerol, 0.1 % sodium azide, Storage buffer may change when conjugated

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    4 °C

    Storage Comment

    Conjugated antibodies should be stored at 4°C
  • Target

    Alginate

    Target Type

    Chemical

    Background

    Sodium alginate is used in biological experiments for the immobilization of cells and encapsulation due to its biocompatibility and simple gelation with divalent cations such as Ca2+. Studies suggest that preparation of alginate microspheres will sustain protein delivery within tissue scaffolds (1), as well as in many other cell types (2).
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