ATP7B antibody (AA 3-21) (PE)

Catalog No. ABIN2485179

This Mouse Monoclonal antibody specifically detects ATP7B in WB, IHC, IF, IP and ICC. It exhibits reactivity toward Human.

Quick Overview for ATP7B antibody (AA 3-21) (PE) (ABIN2485179)

Target: ATP7B (ATPase, Cu++ Transporting, beta Polypeptide (ATP7B))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ATP7B antibody is conjugated to PE

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone: S62-29

Product Details anti-ATP7B Antibody (PE)

Binding Specificity: AA 3-21

Specificity: Detects ~160 kDa in rat brain membrane preparations.

Cross-Reactivity: Human, Mouse, Rat

Purification: Protein G Purified

Immunogen: Synthetic peptide amino acids 3-21 (cytoplasmic N-terminus) of human Copper-transporting ATPase2

Isotype: IgG1

Application Details

Application Notes:

• WB (1:1000)
• ICC/IF (1:100)
• optimal dilutions for assays should be determined by the user.

Comment:

1 μg/ml of ABIN2485179 was sufficient for detection of Copper-transporting ATPase2 in 20 μg of rat brain lysate by colorimetric immunoblot analysis using Goat IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Conjugated antibodies should be stored at 4°C

Target Details for ATP7B

Target: ATP7B (ATPase, Cu++ Transporting, beta Polypeptide (ATP7B))

Alternative Name: ATP7B

Background: The copper efflux transporters ATP7A and ATP7B sequester intracellular copper into the vesicular secretory pathway for export from the cell. ATP7b is an important protein for copper transport and elimination of excess copper from the body. ATP7b transports metals in and out of cells using ATP. There are 3 known isoforms of the ATP7b gene, A is found in the liver, kidney, and brain, the shorter form B is found in brain tissue, and the third isoform, known as WND/140 KDA is found in mitochondria. Mutations in the ATP7b gene can cause Wilson's disease, an inherited disorder causing copper poisoning in the brain and liver, characterized by neurological symptoms and hepatic damage.

Gene ID: 540

NCBI Accession: NP_000044

UniProt: B7ZLR4

Pathways: Transition Metal Ion Homeostasis, Ribonucleoside Biosynthetic Process